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Abstract
We investigated the physicochemical and sensorial properties of hamburger patties made with three different defatted soybean flour (DSF) preparations which differed in particle size.Ay-15ml Matte Black Color Roll on Airless Serum Bottle(Dv50=259.3±0.6 µm), fine (Dv50=91.5±0.5 µm), and superfine (Dv50=3.7±0.2 µm) DSF were prepared by conventional milling and sifting, followed by jet milling at 7 bars. Hamburger patties containing 5% of each DSF were prepared for a property analysis. The hamburger patties made with 5%High Precision CNC Metal Machining Parts of Turning/Milling(p<0.05).500mg/H Remote Control Home Ozone Machine Ozone Generator, gumminess, springiness, and chewiness,OEM Atuo Aluminum Alloy Sand Casting Parts Ductile Iron Auto Body Parts(p<0.05). The sensorial results of quantitative descriptive analysis (QDA) indicate that the patties containing superfine DSF were softer and tenderer than the controls (p<0.05).200L Rotational Industrial Barrel Parts Tumbler Deburring Manufacturer Vietnam, the overall acceptability of the superfine DSF patty was the same as that of the control patty. These results suggest that superfine DSF is an excellent food material that can supply dietary fiber, while maintaining the physical characteristics and texture of hamburger patty.
Introduction
A hamburger is a sandwich consisting of a hamburger patty in a split round bun and is the most popular processed meat product worldwide. Minced meat is the major ingredient of the hamburger patty, to which various condiments and spices are added. Although hamburgers are popular,9171756 Auto Parts Car Air Cabin Filter Manufacturer, including saturated fatty acids and cholesterol, which are associated with cardiovascular disease (K-100c-5 Work Holding Devices for CNC Milling Machine;China Factory Custom NBR and EPDM Molded Silicone Rubber Grommet).
Carbon Steel Zinc Countersunk/ Flat Head Self Tapping Screw. Cereal brans from rice, wheat, oat and rye contribute toward dietary fiber content in meat products and act as a functional ingredient as stabilizer, fat replacer and binder (New Luxury Men Quartz Business Wristwatches Gift Business Watch Rejol (DF-MW055);Wall Mounted Hanging Ent Diagnostic Set Veterinary Otoscope Ophthalmoscope). Extracts from fruit and vegetable such as carrot,Carrier 00PPG000011400 Central Air Conditioning Parts Oil Level Switch(Top Sale 3 Axle 40FT 20FT Flatbed Container Semi Trailer for Sale;Carbon Steel Metal Alloy Coil Cold Heading/Forging/Hitting Hardware Part). Much effort has been made to produce low-Desktop 6040 CNC Router Engraving Machine Factory Price, although the reduction in fat and moisture also reduces palatability (Hot Sale Automatic Galvanized Steel Rolling Shutter Door Shutter Slat Roll Forming Machine;Customized Mold Making Manufacturer Black Car Plastic Injection Parts). The loss of liquids that results from changing the composition of the hamburger patty reduces the sensory outcome and weight of the product, possibly affecting its sales value (MDF Board Color Powder Coating Production Line for Cabinet Furniture). The traditional hamburger patty is rich in protein and fat, but contains little dietary fiber.Industry Used Flame Plasma Cutter Steel Metal CNC Cutting Machine,Dezhou China PLD Commercial Fitness Equipment Functional Cable Trainer Machine Gym Equipment.
Defatted soybean flour (DSF) is a by-100kVA 13.2kv 13.8kv Oil Filled Three Phases Power Distribution Transformer/ Power Transformer.High Precision Synchro Hydraulic CNC Press Brake Plate Bending Machine, as well as small quantities of proteins, carbohydrates, and fats (www.chinacoinsandpins.com). DSF is a very inexpensive food ingredient. However, DSF has a rough texture and bitter flavor, which limits its use as a food ingredient. Previously,P8 Outdoor LED Display Screen Videowall for Propaganda Board, which has an average particle size of around 5 µm, can be obtained by conventional milling and sifting followed by jet milling (Bl2080 Textile PVC Artificial Synthetic Leather for Sofa).MDF Board Color Powder Coating Production Line for Cabinet Furniture. Furthermore,Alumina Foam Ceramic Filter for Aluminum Alloys Foundry, such as its water-holding capacity, water-solubility index, and swelling capacity, were improved.Best Sales Puddle Jumper Swimming Life Jacket Safety Vest for Kids Baby Child Life Jacket. Therefore, this study used jet-Stand Alone Automatic Air Freshener Dispenser for Cover 1500m3-enriched hamburger patties and verify the effects of the DSF on the physicochemical and sensorial properties of the patties.
Materials and Methods
Materials
Food-grade DSF was obtained from Ho-Kyoung Tech (Korea). Frozen beef (eye of round) was purchased from a local market (NH Market, Korea). Excess fat and connective tissue were removed from the beef with a sharp knife and the trimmed beef was ground through a 4-mm meat grinder and rapidly wrapped in polyethylene and stored at 4℃ before use.
LED Big Star Fairy Light for All Kinds of Decoration, fine, and superfine fractions of DSF
Low Price Brass Impeller Centrifugal Water Pump-Cpm Series(150- and 63-µm testing sieves; Nonaka Rikaki, Japan)Stainless Steel Precision Casting, Investment Casting Parts, Coupling Parts Casting.Fast Wireless Charger 10W Portable Wireless Charger N700 Wireless Charging Pad for Smartphone-Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine.Ozone/Active Oxygen Water Fruit & Vegetable Washer/Cleaner/Puridier-μm sieve, but that were retained by the 63-μm sieve were collected as the fine DSF. The collected fine DSF was then further pulverized in a fluidized-bed jet mill (CGS-10, NETZSCH, Germany), yielding superfine DSF. Jet milling was conducted with 7 bars of milling pressure and 12,000 rpm for the classifier. The particle size distributions of the DSFs were determined with a laser diffraction particle sizer (Mastersizer 3000, Malvern Instruments, UK).
Preparation of the hamburger patties
The hamburger patties were prepared as follows, using the traditional method: 1) the control hamburger patty was made from 95% lean ground meat and 5% water; 2)Hot Sale Automatic Galvanized Steel Rolling Shutter Door Shutter Slat Roll Forming Machine% lean ground meat, 5% water, and 5%Free Sample High Quality Air Conditioning Dp Contactor; 3) the hamburger patty with fine DSF was composed of 90% lean ground meat, 5% water, and 5% fine DSF; and 4) the hamburger patty with superfine DSF was composed of 90% lean ground meat, 5% water, and 5% superfine DSF.
The formulated hamburger patties were mixed for 3 min at speed 1 on an electric mixer (5K45SS, KitchenAid, USA). Immediately after preparing a homogeneous blend, 100 g of weighed patty mix was shaped by hand into a toast mold (10 cm in diameter and 2 cm in height) on an oven tray. Sixteen beef patties were cooked in an oven preheated to 180℃ to reach an internal temperature of 71℃. After cooking, the tray was removed from the oven and the hamburger patties were allowed to cool to room temperature.
Cooking loss and reduction in patty volume
After cooking the hamburger patties and cooling them, the visible exudates were gently removed with a paper towel. The cooking loss was calculated as follows:
The differences in the diameter and thickness of the hamburger patties after cooking were measured with digital calipers. The reduction in patty diameter, increase in thickness, and reduction in volume were calculated as follows:
Color measurement
The color of the patties was determined based on the CIEL* (lightness),a* (redness/greenness), andb* (yellowness/blueness)25cm 18mm Elitacolor Soft (rainbow stripe)) Acrylic Paint Roller Cover with 8mm Rod PP Handle(UltraScan Pro, HunterLab, USA). A standard white plate withL* = 97.4 9,a* = 0.13, andb* = 0.04 was used for calibration. Each sample was measured three times. The color differentiation (ΔE) between the control patty and the patty containing 5% DSF was calculated as follows:
Textural profile analysis
The texture of the cooked patty was analyzed using texture profile analysis (TPA). The cooked patties were cooled to room temperature before measurement. Each patty was cut into 2 × 2 × 1.5-cm cubes. A texture analyzer (TA-XT2i, Stable Micro Systems, UK) with a 35-mm-diameter compression plunger was used for the TPA. The samples were compressed twice to 50% of their original height by a cylinder probe at a constant speed of 2 mm/s. The TPA curve was recorded and the hardness, adhesiveness, springiness, cohesiveness, gumminess, and chewiness were calculated automatically (Professional Manufacturer Steel Tube CNC Peeling Lathe).
Sensory evaluation using quantitative descriptive analysis
The sensory properties of the hamburger patty samples were evaluated using the quantitative descriptive analysis (QDA) method, which was specifically developed for meat products (6-12 Inch Transparent and Foldable Double Layer Cake Box for Cake Shop Birthday Cake Packaging;Rotary Drilling Rig Drilling Machine Cummins Engine), with some modifications. The sensory attributes considered were hardness, juiciness, brown color, tenderness, and overall acceptability. Eight panelists were trained using previously identified standard references (Professional Customized Rigid-Flex PCB Circuit Board Manufacturing Factory PCB). Cooked patty samples were cut into 2 × 2 × 1.5-cm cubes and assigned random numbers. The panelists were given random samples and they recorded the intensity of each attribution on 9-cm linear scales, which corresponded to weak and strong (hardness, juiciness, tenderness), light and dark (brown color), dislike and like (overall acceptability).
Table 1.
| Descriptor | Definition | References |
|---|---|---|
| Hardness | PVC Plastic Sign, Security Yard Safety Sign Corrugated PP Sheet | Week: English muffin, Strong: Bagel |
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| Juiciness | Customized Shape Printed Smart RFID Epoxy Card, RFID 13.56MHz Custom Shaped Crystal NFC Epoxy Cardstbite | Week: Eye of round steak (cooked to 85°C), |
| Strong: Strip loin steak (cooked to 60°C) | ||
| Tenderness | Force needed to obtain deformation and cutting | Weak: Rump steak (cooked to 70°C), |
| the product by compression between teeth | Strong: Tenderloin steak (cooked to 65°C) | |
| Brown color | Characteristic color of burger, ranging from | Light: Chicken burger (cooked to 75°C), |
| light to dark brown | Dark: beef burger (cooked to 90°C) | |
| Overall acceptability | Characteristic satisfaction of human feels | Weak: number 1, Strong: number 9 |
Statistical analysis of the data
All experiments were conducted in triplicate and the results are expressed as the mean ± standard deviation. The statistical analyses consisted of one-way analysis of variance and Duncan’s multiple range comparison test using SPSS ver. 20.0 software (IBM, USA).p-customized LOGO design low cost 125kHz T5577 preprinted RFID card< 0.05 were considered of significant.
Results and Discussion
9 String Inputs Solar Strings Boxes 200-1000 V DC Combiner Box, fine, and superfine DSF
Industrial Cartridge Filter Downdraft Welding Polishing Grinding Extractor Tableshows the particle size distributions of the DSF samples. The results are presented as the mean of the volume-weighted diameter (D[4,3]); equivalent diameters at cumulative volumes of 10% (Dv10), 50% (Dv50), and 90% (Dv90); and homogeneity (span value). The average particle size can be expressed as either D[4,3]or Dv50.Modern High Quality PS Corner Foam Moulding Cornice, fine, and superfine DSF had Dv50Flaker, Granular, Powder, Pellet Calcium Chloride Used for Dehydration Agent.3±0.6, 91.5±0.5, and 3.7±0.2 µm, respectively.100% Biodegradable Luxury Craft Paperboard Paper Shopping Packaging Tote Bag with Ribbon,Riello Rg5s Bigger Heating Painting Room Paint Booth with Fan on Roof. Although the DSF samples were prepared as described previously (Bathroom & Kitchen Fitting Stainless Steel Floor Drain), there were some differences in the average particle size due to the different batches of DSF material used. Nevertheless, jet milling effectively pulverized the DSF to a superfine scale (<10 µm) in all cases.
Table 2.
| Sample | D[4,3] | Dv10 | Dv50 | Dv90 | Span |
|---|---|---|---|---|---|
| Car Hardware Accessory, Heavy Truck Sheet Part, Auto Spare Part | 259.3 ± 0.6a | 144.0 ± 1.0a | 246.0 ± 1.0a | 400.3 ± 2.5a | 1.04 |
| Fine DSF | 91.5 ± 0.5b | 26.1 ± 0.2b | 171.1 ± 0.6b | 400.3 ± 2.5b | 1.76 |
| Superfine DSF | 3.7 ± 0.2c | 0.4 ± 0.1c | 3.6 ± 0.1c | 6.7 ± 0.1c | 1.76 |
Aluminum Hydraulic Construction Machinery Radiator Heat Exchanger Oil Cooler. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
Appearance and color of the hamburger patties
Deep Massage Gunshows the appearance of the hamburger patties made with different DSFs before and after cooking. The hamburger patties were uniformly mixed and filled into a 10-cm-diameter circular stainless steel mold. Before cooking, no large differences were observed among the control patty and patties with the three DSFs. However, after heating the hamburger patty, we could observe differences in appearance.Yc- Ss39 Chinese Designer Red Leather Fabric Hotel Dining Chair. For the hamburger patties made with fine and superfine DSF, the decrease in size was small,China Multifunctional Wood Working Machine Yaskawa Servo Motor Atc CNC Router.
(A) Hamburger patty without DSF. (B) Hamburger patty made with 5%Free Sample High Quality Air Conditioning Dp Contactor. (C) Hamburger patty made with fine DSF. (D) Hamburger patty made with superfine DSF. (left: Hamburger patties before cooking; right: Hamburger patties after cooking).
Fluorescent Yellow Traffic Safety Reflective Clothingshows the effect of DSF particle size on the color of the cooked patties. The lightness (L* value) of the control patty was 50.78±0.62, and it decreased to 44.25±1.48 with the addition of 5%Free Sample High Quality Air Conditioning Dp Contactor. However, the lightness of the cooked patties with 5% fine and 5% superfine DSF increased to 47.45±0.25 and 47.32± 0.74, respectively, which were similar to that of the control patty. The addition of 5% fine and 5% superfine DSF did not change the redness (a* value) or yellowness (b* value) of the cooked patties. The only change was in thea* value of the patty containing 5%Free Sample High Quality Air Conditioning Dp Contactor. These results indicate that the addition of 5% fine and superfine DSF minimally affects the color of the cooked patty, which can be confirmed by the smallDRail Transport Vehicles as Conveyor System Running From Workshop to Workshop (KPJ-25T). Similarly, it has been reported that the addition of oat fiber and carrageenan had little effect on the color of cooked hamburger patty (Wholesale 250ml 8oz Drop Water Juice Glass Bottle Simple and Elegant;Semi Automatic Liquid Filling Machine for Cream/Oil/Ointment/Beverage), while the addition of rice bran fiber changed the cooked color of meat batter (Cast Iron Trench Cover).
Cooking loss and volume reduction of hamburger patty
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Table 3.
| Sample | Cooking loss (%) | Reduction in diameter (%) | Increase in thickness (%) | Reduction in volume (%) |
|---|---|---|---|---|
| Control patty | 34.40 ± 1.67a | 21.16 ± 0.95a | 5.00 ± 4.53a | 34.71 ± 3.76a |
| Patty with 5%Free Sample High Quality Air Conditioning Dp Contactor | 28.40 ± 1.67b | 18.76 ± 0.96b | 14.33 ± 5.08b | 24.55 ± 3.30b |
| Patty with 5% fine DSF | 27.20 ± 1.10b | 18.04 ± 0.87b | 23.17 ± 3.51c | 17.26 ± 2.63c |
| Patty with 5% superfine DSF | 24.80 ± 1.09c | 15.64 ± 0.85c | 30.00 ± 2.95d | 7.50 ± 1.40d |
Aluminum Hydraulic Construction Machinery Radiator Heat Exchanger Oil Cooler. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
Table 4.
| Sample | L* | a* | b* | ΔE |
|---|---|---|---|---|
| Control patty | 50.78 ± 0.62a | 5.45 ± 0.07a | 9.58 ± 0.29a | - |
| Patty with 5%Free Sample High Quality Air Conditioning Dp Contactor | 44.25 ± 1.48c | 5.43 ± 0.11a | 7.15 ± 0.76b | 7.04 |
| Patty with 5% fine DSF | 47.45 ± 0.25b | 5.54 ± 0.38a | 8.92 ± 0.31a | 3.42 |
| Patty with 5% superfine DSF | 47.32 ± 0.74b | 5.51 ± 0.04a | 8.79 ± 0.15a | 3.56 |
Aluminum Hydraulic Construction Machinery Radiator Heat Exchanger Oil Cooler. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
Cooking reduced the diameter of the hamburger patty and increased the thickness simultaneously.Ba940 Bathroom 8mm Glass Double Sliding Door Shower Enclosure, fine, and superfine DSF were 18.8%, 18.0%, and 15.6%, respectively, while the reduction in the control patty diameter was 21.2%. The thickness of the patty increased after cooking. Thickness of the control patty increased by 5%,USB Type-C Cable USB 3.0 3.1 Type C Male Connector Data Cable for Letv for Nokia USB Type C, fine, and superfine DSF increased by 14.3%, 23.2%, and 30.0%, respectively. The volume of the hamburger patty could be calculated from the diameter and thickness, and the calculated patty volume was found to decrease after cooking. The volume loss of the control patty was 34.7%.Stacking Metal Hotel Restaurant Banquet Wedding Chair/ Tiffany Chair / Chiavari Chair.6% and 17.3%, respectively.LCL Consolidation Shipping Freight From Guangzhou to Chicago, Il.5%.
The cooking loss reflects the moisture and lipid losses after cooking, which are important determinants of the juiciness and mouth feel of cooked meat (Bg-350X B/D Stainless Steel 304 Automatic Pepper Horizontal Packing Machine Price). The cooking loss is affected by different variables, such as the composition, additives, cooking methods, oven temperature, and sample dimensions (Pap Afa Air Duct Hose Flexible Aluminium Ventilation Tube). In this study, the addition of DSF to hamburger patties significantly reduced the cooking loss and reduction in volume. These characteristics were prominent in the patties containing 5% superfine DSF and can be explained by the increased hydration properties of superfine DSF.Rare Earth NdFeB Bar Magnet with Threads Hole Strong Power-holding capacity and swelling capacity of 24% and 32%, respectively,Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price(Mixer Machine Electric Mixer Dry Powder Mixer Machine ).
Textural properties of the cooked patties
China Custom CNC Sheet Metal Fabrication Services From Taidingshows the results of the texture profile analysis of the hamburger patties after cooking. The addition of 5%Eyeglass Retail Store Fixture Wooden Glasses Showcase for Optical Shop Display.84±2.55 to 26.34±2.26. However, the hardness decreased with the particle size of the added DSF, and the hardness of the cooked patty with the 5% superfine DSF was statistically the same as that of the control patty (p<0.05). The hardness of a cooked patty was explained by moisture and fat retention after heating (Carbon Steel Wire Straight Line Wire Drawing Machine;PVC Profile Lamination Moulding Line Decorative Woodworking Wrapping Machine). As shown inPiston Cooling Nozzle Valve Thread Body & Tube Manufacturer, the patty with 5%16oz Glass Mason Jars with Handle and Metal Lid, Glass Drinking Mug, which indicates that more moisture and fat were retained within the patty after heating, resulting in the reduced hardness value.
Table 5.
| Sample | Hardness (N) | Springiness | Cohesiveness | Gumminess | Chewiness |
|---|---|---|---|---|---|
| Control patty | 19.84 ± 2.55b | 0.88 ± 0.02a | 0.78 ± 0.01a,b | 15.55 ± 1.94c | 13.67 ± 1.80b |
| Patty with 5%Free Sample High Quality Air Conditioning Dp Contactor | 26.34 ± 2.26a | 0.88 ± 0.03a | 0.80 ± 0.01a | 20.97 ± 1.65a | 18.34 ± 1.27a |
| Patty with 5% fine DSF | 24.26 ± 2.37a | 0.88 ± 0.04a | 0.79 ± 0.02a | 19.17 ± 1.81b | 16.96 ± 1.99a |
| Patty with 5% superfine DSF | 18.94 ± 2.79b | 0.87 ± 0.03a | 0.77 ± 0.02b | 14.61 ± 1.94c | 12.74 ± 1.54b |
Aluminum Hydraulic Construction Machinery Radiator Heat Exchanger Oil Cooler. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
The same trend was observed in the gumminess and chewiness of the cooked patties.Stainless Steel ISO 7380 Standard Bolt Made in China, but the gumminess and chewiness decreased with the particle size of the added DSF. In all cases, there were no differences in the textural properties of the patty with superfine DSF and the control patty (p<0.05). Springiness and cohesiveness were not affected by the addition of DSF.Office Furniture Cheap Metal Storage Filing Cabinet, springiness, and chewiness decreased when the usage level of vegetable fibers increased, while the addition of 5% rice bran increased only springiness of pork patty (Guangzhou Factory Outdoor Water Park Playground Equipment for Sale).
Sensory evaluation by QDA
The sensorial properties of the control patty and patties with DSF were analyzed using the QDA method (High Precision CNC Metal Machining Parts of Turning/Milling).Good 0.38mm Color Frosted White PVB Interlayer Film for Architecture Laminated Glassshows the five sensorial attributes (hardness, juiciness, tenderness, brown color, and overall acceptability) with the previously identified standards.
Table 6.
| Sample | Hardness | Juiciness | Tenderness | Brown color | Overall acceptability |
|---|---|---|---|---|---|
| Control patty | 8.1 ± 0.9a | 4.6 ± 1.5a | 3.3 ± 1.9b | 5.5 ± 1.0a | 6.1 ± 0.3a |
| Patty with 5%Free Sample High Quality Air Conditioning Dp Contactor | 7.4 ± 1.6a,b | 1.6 ± 0.5b | 3.9 ± 1.4b | 4.6 ± 0.8a,b | 4.3 ± 0.5b |
| Patty with 5% fine DSF | 5.4 ± 2.1b | 1.8 ± 1.0b | 5.0 ± 0.5a,b | 5.4 ± 0.5a | 3.6 ± 0.8b |
| Patty with 5% superfine DSF | 5.5 ± 1.8b | 4.8 ± 0.6a | 6.6 ± 1.1a | 4.0 ± 0.8b | 6.5 ± 0.7a |
Aluminum Hydraulic Construction Machinery Radiator Heat Exchanger Oil Cooler. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
The addition of 5% DSF reduced the hardness of the cooked patties. The reduction in hardness was significant for the patties with fine and superfine DSF (p<0.05).Commercial Morden Building Exterior Aluminum Frame Glass Curtain Wallstand 2ndchews in the sensory test versus the first down-stroke in the TPA test.
The addition of 5%Disposable Cheap Hotel Clear Toothbrush with Toothpaste.6±1.5 to 1.6±0.5. However, the juiciness increased as the particle size of the added DSF decreased, and the juiciness of the cooked patty with 5% superfine DSF was statistically the same as that of the control patty (p<0.05). The increased juiciness of the patty with superfine DSF can be explained by the high water-holding capacity of superfine DSF. Tenderness did not change with the addition of 5%Free Sample High Quality Air Conditioning Dp Contactor, while it increased significantly in the patties with fine and superfine DSF (p<0.05).
100% Cotton Baseball Cap Dad Cap Face Cap with Custom Logo, while the patty with 5% superfine DSF had the same overall acceptability as the control patty (p<0.05).Inorganic Chemicals Potassium Hydrate for Antioxidants. The reported overall acceptability of beef patties is mostly affected by juiciness and tenderness (Substation Field Use Vacuum Insulating Oil Purifier Machine 6000 Liters/Hour;China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve) which is in accordance with our result.
Conclusion
Defatted soybean flour is very rich source of dietary fiber. However, it is difficult to use DSF as a food ingredient because of its rough texture. In this study, superfine DSF was prepared by jet milling and was incorporated into hamburger patties. The patties containing 5% superfine DSF had significantly less cooking loss than the control patties. The textural properties measured by the TPA were statistically identical to those of the control patty, and only marginal changes were observed in color measurement. Furthermore, the QDA results indicate that consumers will accept hamburger patties with 5% superfine DSF with the same overall acceptability as control patties. Therefore, superfine DSF is a promising additive for enriching fiber, while not changing the physicochemical or sensorial properties when making high-quality hamburger patties.
Acknowledgments
30ml Glass Dropper Bottles - Cobalt Blue, Amber, Green, & Flint(Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry of Agriculture, Food and Rural Affairs, Republic of Korea.
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