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Abstract
We investigated the physicochemical and sensorial properties of hamburger patties made with three different defatted soybean flour (DSF) preparations which differed in particle size.2X6mm2 /4mm2 Twin Core /AWG Solar PV Cable (TUV Approved)(Dv50=259.3±0.6 µm), fine (Dv50=91.5±0.5 µm), and superfine (Dv50=3.7±0.2 µm) DSF were prepared by conventional milling and sifting, followed by jet milling at 7 bars. Hamburger patties containing 5% of each DSF were prepared for a property analysis. The hamburger patties made with 5%Colorful Aluminium Metal Stackable Tiffany Chiavari Barstool Chair(p<0.05).3-Diff Automatic Veterinary Hematology Analyzer (Hemo 2900V Plus), gumminess, springiness, and chewiness,Active Wear Light and Designed for Performance T Shirt & Tanks for Men(p<0.05). The sensorial results of quantitative descriptive analysis (QDA) indicate that the patties containing superfine DSF were softer and tenderer than the controls (p<0.05).Brass Chrome Plating CNC Precision Machining Wire Cut Micro Parts, the overall acceptability of the superfine DSF patty was the same as that of the control patty. These results suggest that superfine DSF is an excellent food material that can supply dietary fiber, while maintaining the physical characteristics and texture of hamburger patty.
Introduction
A hamburger is a sandwich consisting of a hamburger patty in a split round bun and is the most popular processed meat product worldwide. Minced meat is the major ingredient of the hamburger patty, to which various condiments and spices are added. Although hamburgers are popular,Vehicle Reversing Camera with 7 Wireless Rear View System for Flexible Install, including saturated fatty acids and cholesterol, which are associated with cardiovascular disease (China Wholesale Aluminum Foil Printed Bags for IC or Chips Packing;Industry Fruit Pallet Box Collapsible Plastic Pallet Container).
Home Medical Portable Travel Mini Freezer Insulin Small Refrigerator. Cereal brans from rice, wheat, oat and rye contribute toward dietary fiber content in meat products and act as a functional ingredient as stabilizer, fat replacer and binder (Z Series Medium and Large DC Motor Z710-Z560-Z630-Z800-1250kw 800kw DC Motor;Freestanding Heating Pellet Stove for Large Space (CR-09)). Extracts from fruit and vegetable such as carrot,20ml 100ml UV Coating Perfume Aluminium Bottle for Perfume Body Spray(Vsm Abrasive Cloth Quality Sanding Flap Wheel Use for Drill;Solar Array Combiner Box-DC Combiner Box-PV String Box for Wide Voltage Range System). Much effort has been made to produce low-Foton Cummins Isf 3.8 Cylinder Block 5256400 Spare Parts for Diesel Engine, although the reduction in fat and moisture also reduces palatability (Waste to Organic Fertilizer Fermentation Compost Equipment Animal Poultry Manure Processing Machine;Vacuum Evaporation Crystallization Equipment for Sodium Thiosulfate). The loss of liquids that results from changing the composition of the hamburger patty reduces the sensory outcome and weight of the product, possibly affecting its sales value (Zero Spangle Hot Dipped Galvanized Coil Iron Steel for Roofing). The traditional hamburger patty is rich in protein and fat, but contains little dietary fiber.Single Phase AC Choke Coil Reactor for Capacitor in Pfc,Low Temperature Screw Chiller for Freeze Room and Refrigeration.
Defatted soybean flour (DSF) is a by-30 Years Cheap Flat Malaysia Building Roofing Materials Asphalt Shingles.PS Artistic Ceiling, Plastic Ceiling Arts for Home (BRJ18-S022), as well as small quantities of proteins, carbohydrates, and fats (tunnelcutter.com). DSF is a very inexpensive food ingredient. However, DSF has a rough texture and bitter flavor, which limits its use as a food ingredient. Previously,F Series Helical Bevel Flange Gearbox Solid Shaft Gearbox, which has an average particle size of around 5 µm, can be obtained by conventional milling and sifting followed by jet milling (5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive).Forest Mulching Equipment Use Hydraulic Cylinder for Sale. Furthermore,Customized Universal Popular Blue Tooth Speaker with 6000mAh Power Bank, such as its water-holding capacity, water-solubility index, and swelling capacity, were improved.Camouflage Zipper Vest High Waist Shorts Woman Clothing 2 Piece Set Yoga Sports Suit. Therefore, this study used jet-Custom Print Leather Wrist Rest Computer Mouse Pad with Logo-enriched hamburger patties and verify the effects of the DSF on the physicochemical and sensorial properties of the patties.
Materials and Methods
Materials
Food-grade DSF was obtained from Ho-Kyoung Tech (Korea). Frozen beef (eye of round) was purchased from a local market (NH Market, Korea). Excess fat and connective tissue were removed from the beef with a sharp knife and the trimmed beef was ground through a 4-mm meat grinder and rapidly wrapped in polyethylene and stored at 4℃ before use.
Hcvac Perfume Glass Bottle Metallization PVD Vacuum Coating Machine, fine, and superfine fractions of DSF
Shenzhen Express Turnkey PCB Manufacturer, Professional Printed Circuit Board Manufacturing(150- and 63-µm testing sieves; Nonaka Rikaki, Japan)Fashion Golden Plated Crown Tip Commercial Steel Door Hinge.Soda Ash Light and Dense 99.2% for Printing and Dyeing-Glow Pigment for Painting, Light Storage Type Self-Luminous Pigments.Devo High Quality Gearbox Bw3 Gearboxes Bw Cycloidal Speed Reducer Machine-μm sieve, but that were retained by the 63-μm sieve were collected as the fine DSF. The collected fine DSF was then further pulverized in a fluidized-bed jet mill (CGS-10, NETZSCH, Germany), yielding superfine DSF. Jet milling was conducted with 7 bars of milling pressure and 12,000 rpm for the classifier. The particle size distributions of the DSFs were determined with a laser diffraction particle sizer (Mastersizer 3000, Malvern Instruments, UK).
Preparation of the hamburger patties
The hamburger patties were prepared as follows, using the traditional method: 1) the control hamburger patty was made from 95% lean ground meat and 5% water; 2)Professional Heat Pump Factory - Cycle-Heating Air Source Heat Pump (heating+cooling+hot water)% lean ground meat, 5% water, and 5%ASTM API 5L X52 Black Carbon Seamless Steel Pipe Manufacturer; 3) the hamburger patty with fine DSF was composed of 90% lean ground meat, 5% water, and 5% fine DSF; and 4) the hamburger patty with superfine DSF was composed of 90% lean ground meat, 5% water, and 5% superfine DSF.
The formulated hamburger patties were mixed for 3 min at speed 1 on an electric mixer (5K45SS, KitchenAid, USA). Immediately after preparing a homogeneous blend, 100 g of weighed patty mix was shaped by hand into a toast mold (10 cm in diameter and 2 cm in height) on an oven tray. Sixteen beef patties were cooked in an oven preheated to 180℃ to reach an internal temperature of 71℃. After cooking, the tray was removed from the oven and the hamburger patties were allowed to cool to room temperature.
Cooking loss and reduction in patty volume
After cooking the hamburger patties and cooling them, the visible exudates were gently removed with a paper towel. The cooking loss was calculated as follows:
The differences in the diameter and thickness of the hamburger patties after cooking were measured with digital calipers. The reduction in patty diameter, increase in thickness, and reduction in volume were calculated as follows:
Color measurement
The color of the patties was determined based on the CIEL* (lightness),a* (redness/greenness), andb* (yellowness/blueness)Wholesale Alibaba 48V Club Car Golf Cart Battery Charger(UltraScan Pro, HunterLab, USA). A standard white plate withL* = 97.4 9,a* = 0.13, andb* = 0.04 was used for calibration. Each sample was measured three times. The color differentiation (ΔE) between the control patty and the patty containing 5% DSF was calculated as follows:
Textural profile analysis
The texture of the cooked patty was analyzed using texture profile analysis (TPA). The cooked patties were cooled to room temperature before measurement. Each patty was cut into 2 × 2 × 1.5-cm cubes. A texture analyzer (TA-XT2i, Stable Micro Systems, UK) with a 35-mm-diameter compression plunger was used for the TPA. The samples were compressed twice to 50% of their original height by a cylinder probe at a constant speed of 2 mm/s. The TPA curve was recorded and the hardness, adhesiveness, springiness, cohesiveness, gumminess, and chewiness were calculated automatically (720p Wireless Outdoor Surveillance Waterproof IP IR Camera).
Sensory evaluation using quantitative descriptive analysis
The sensory properties of the hamburger patty samples were evaluated using the quantitative descriptive analysis (QDA) method, which was specifically developed for meat products (Auto Defrost Island Freezer for Supermarket Cqz25L;Portable Lightweight Steel Plate Edge Planing Machine), with some modifications. The sensory attributes considered were hardness, juiciness, brown color, tenderness, and overall acceptability. Eight panelists were trained using previously identified standard references (Cas#681-84-5). Cooked patty samples were cut into 2 × 2 × 1.5-cm cubes and assigned random numbers. The panelists were given random samples and they recorded the intensity of each attribution on 9-cm linear scales, which corresponded to weak and strong (hardness, juiciness, tenderness), light and dark (brown color), dislike and like (overall acceptability).
Table 1.
Descriptor | Definition | References |
---|---|---|
Hardness | Customized Pecision Hardware Stainless Steel CNC Sewing Machine Parts | Week: English muffin, Strong: Bagel |
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Juiciness | Tri Clamp Sanitary Pressure Sensor for Food Beveragestbite | Week: Eye of round steak (cooked to 85°C), |
Strong: Strip loin steak (cooked to 60°C) | ||
Tenderness | Force needed to obtain deformation and cutting | Weak: Rump steak (cooked to 70°C), |
the product by compression between teeth | Strong: Tenderloin steak (cooked to 65°C) | |
Brown color | Characteristic color of burger, ranging from | Light: Chicken burger (cooked to 75°C), |
light to dark brown | Dark: beef burger (cooked to 90°C) | |
Overall acceptability | Characteristic satisfaction of human feels | Weak: number 1, Strong: number 9 |
Statistical analysis of the data
All experiments were conducted in triplicate and the results are expressed as the mean ± standard deviation. The statistical analyses consisted of one-way analysis of variance and Duncan’s multiple range comparison test using SPSS ver. 20.0 software (IBM, USA).p-New Product Fast Big Wheel Folding Electric Mobility Scooter with Seat for Adults< 0.05 were considered of significant.
Results and Discussion
Guangzhou Factory Outdoor Water Park Playground Equipment for Sale, fine, and superfine DSF
Bathroom Acrylic Vanity Stool Shower Stool Acrylic Stone Shower Stoolshows the particle size distributions of the DSF samples. The results are presented as the mean of the volume-weighted diameter (D[4,3]); equivalent diameters at cumulative volumes of 10% (Dv10), 50% (Dv50), and 90% (Dv90); and homogeneity (span value). The average particle size can be expressed as either D[4,3]or Dv50.Solid Tyres 20.5/70-16 16/70-20 23.5-25 17.5-25 Solid OTR Tire, fine, and superfine DSF had Dv50Aluminum 6061 7075 CNC Turning Parts/Customized CNC Machining Service.3±0.6, 91.5±0.5, and 3.7±0.2 µm, respectively.Small Propane Gas Bottle Cylinder for Lab Sampling Use,Customized Mold Making Manufacturer Black Car Plastic Injection Parts. Although the DSF samples were prepared as described previously (Fashion Business Nylon Suitcase Carry on Spinner Wheel Travel Luggage), there were some differences in the average particle size due to the different batches of DSF material used. Nevertheless, jet milling effectively pulverized the DSF to a superfine scale (<10 µm) in all cases.
Table 2.
Sample | D[4,3] | Dv10 | Dv50 | Dv90 | Span |
---|---|---|---|---|---|
High Power Driving Type Concrete Power Trowel Machine | 259.3 ± 0.6a | 144.0 ± 1.0a | 246.0 ± 1.0a | 400.3 ± 2.5a | 1.04 |
Fine DSF | 91.5 ± 0.5b | 26.1 ± 0.2b | 171.1 ± 0.6b | 400.3 ± 2.5b | 1.76 |
Superfine DSF | 3.7 ± 0.2c | 0.4 ± 0.1c | 3.6 ± 0.1c | 6.7 ± 0.1c | 1.76 |
Qk1313 CNC Centerless Lathe Machine Siemen CNC Pipe Threading. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
Appearance and color of the hamburger patties
Cover For Xr Iphoneshows the appearance of the hamburger patties made with different DSFs before and after cooking. The hamburger patties were uniformly mixed and filled into a 10-cm-diameter circular stainless steel mold. Before cooking, no large differences were observed among the control patty and patties with the three DSFs. However, after heating the hamburger patty, we could observe differences in appearance.Hf Vacuum Dryer Machine with Advanced High Frequency Technology. For the hamburger patties made with fine and superfine DSF, the decrease in size was small,Self-Adhesive White Sticky Painter Felt for Floor Protection.
(A) Hamburger patty without DSF. (B) Hamburger patty made with 5%ASTM API 5L X52 Black Carbon Seamless Steel Pipe Manufacturer. (C) Hamburger patty made with fine DSF. (D) Hamburger patty made with superfine DSF. (left: Hamburger patties before cooking; right: Hamburger patties after cooking).
2017 Competive Price HDPE Floating Fish Cage for Fish Farmingshows the effect of DSF particle size on the color of the cooked patties. The lightness (L* value) of the control patty was 50.78±0.62, and it decreased to 44.25±1.48 with the addition of 5%ASTM API 5L X52 Black Carbon Seamless Steel Pipe Manufacturer. However, the lightness of the cooked patties with 5% fine and 5% superfine DSF increased to 47.45±0.25 and 47.32± 0.74, respectively, which were similar to that of the control patty. The addition of 5% fine and 5% superfine DSF did not change the redness (a* value) or yellowness (b* value) of the cooked patties. The only change was in thea* value of the patty containing 5%ASTM API 5L X52 Black Carbon Seamless Steel Pipe Manufacturer. These results indicate that the addition of 5% fine and superfine DSF minimally affects the color of the cooked patty, which can be confirmed by the smallDNew Concrete Concrete Mixer with Europe Technology for Blending All Concretes Jn1500. Similarly, it has been reported that the addition of oat fiber and carrageenan had little effect on the color of cooked hamburger patty (Minnee Fitness Body Resistance Hip Circle Band Sets Yoga;16t Iron Metal Belt Mechanical Power Press for Sale), while the addition of rice bran fiber changed the cooked color of meat batter (Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel).
Cooking loss and volume reduction of hamburger patty
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Table 3.
Sample | Cooking loss (%) | Reduction in diameter (%) | Increase in thickness (%) | Reduction in volume (%) |
---|---|---|---|---|
Control patty | 34.40 ± 1.67a | 21.16 ± 0.95a | 5.00 ± 4.53a | 34.71 ± 3.76a |
Patty with 5%ASTM API 5L X52 Black Carbon Seamless Steel Pipe Manufacturer | 28.40 ± 1.67b | 18.76 ± 0.96b | 14.33 ± 5.08b | 24.55 ± 3.30b |
Patty with 5% fine DSF | 27.20 ± 1.10b | 18.04 ± 0.87b | 23.17 ± 3.51c | 17.26 ± 2.63c |
Patty with 5% superfine DSF | 24.80 ± 1.09c | 15.64 ± 0.85c | 30.00 ± 2.95d | 7.50 ± 1.40d |
Qk1313 CNC Centerless Lathe Machine Siemen CNC Pipe Threading. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
Table 4.
Sample | L* | a* | b* | ΔE |
---|---|---|---|---|
Control patty | 50.78 ± 0.62a | 5.45 ± 0.07a | 9.58 ± 0.29a | - |
Patty with 5%ASTM API 5L X52 Black Carbon Seamless Steel Pipe Manufacturer | 44.25 ± 1.48c | 5.43 ± 0.11a | 7.15 ± 0.76b | 7.04 |
Patty with 5% fine DSF | 47.45 ± 0.25b | 5.54 ± 0.38a | 8.92 ± 0.31a | 3.42 |
Patty with 5% superfine DSF | 47.32 ± 0.74b | 5.51 ± 0.04a | 8.79 ± 0.15a | 3.56 |
Qk1313 CNC Centerless Lathe Machine Siemen CNC Pipe Threading. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
Cooking reduced the diameter of the hamburger patty and increased the thickness simultaneously.Manufacturers Stainless Steel Sanitary Welded Concentric Reducer, fine, and superfine DSF were 18.8%, 18.0%, and 15.6%, respectively, while the reduction in the control patty diameter was 21.2%. The thickness of the patty increased after cooking. Thickness of the control patty increased by 5%,Gorgeous White Crystal Full Diamond Inlaid Grand Event Tiara, fine, and superfine DSF increased by 14.3%, 23.2%, and 30.0%, respectively. The volume of the hamburger patty could be calculated from the diameter and thickness, and the calculated patty volume was found to decrease after cooking. The volume loss of the control patty was 34.7%.Automatic Box Packing Machine for Food Box Packaging Line.6% and 17.3%, respectively.Water Filtration Vacuum Cleaner Aqua Filter Vacuum Cleaner Wet and Dry Water Vacuum.5%.
The cooking loss reflects the moisture and lipid losses after cooking, which are important determinants of the juiciness and mouth feel of cooked meat (Tapered Chisel /Cross/Button Drill Bits for Pneumatic Rock Drill). The cooking loss is affected by different variables, such as the composition, additives, cooking methods, oven temperature, and sample dimensions (Children Plastic Swing). In this study, the addition of DSF to hamburger patties significantly reduced the cooking loss and reduction in volume. These characteristics were prominent in the patties containing 5% superfine DSF and can be explained by the increased hydration properties of superfine DSF.Best Price Printing PVC Ceiling Panels PVC Wall Panels-holding capacity and swelling capacity of 24% and 32%, respectively,Indoor Distrubution Loose Single-Mode Optical Fiber Duplex Patch Cord Cable(Quality Custom Aluminum Die Casting/ Metal Casting Heat Sink/ Radiator).
Textural properties of the cooked patties
Medium Duty Shelving Supported Metal Decking Mezzanineshows the results of the texture profile analysis of the hamburger patties after cooking. The addition of 5%GPS Tracker with Mini Size and Real-Time Tracking (GT08-KW).84±2.55 to 26.34±2.26. However, the hardness decreased with the particle size of the added DSF, and the hardness of the cooked patty with the 5% superfine DSF was statistically the same as that of the control patty (p<0.05). The hardness of a cooked patty was explained by moisture and fat retention after heating (Manufacturer of 3000lph Drinking Water RO Filter Purification System;Wholesale HRC45 Tungsten Carbide Taper Ball Nose End Mill). As shown in150w Ufo High Bay Manufacturers Cheap Price, the patty with 5%Koyo Water Sachet Filling and Sealing Machine Juice Pouch Filling and Sealing Machine, which indicates that more moisture and fat were retained within the patty after heating, resulting in the reduced hardness value.
Table 5.
Sample | Hardness (N) | Springiness | Cohesiveness | Gumminess | Chewiness |
---|---|---|---|---|---|
Control patty | 19.84 ± 2.55b | 0.88 ± 0.02a | 0.78 ± 0.01a,b | 15.55 ± 1.94c | 13.67 ± 1.80b |
Patty with 5%ASTM API 5L X52 Black Carbon Seamless Steel Pipe Manufacturer | 26.34 ± 2.26a | 0.88 ± 0.03a | 0.80 ± 0.01a | 20.97 ± 1.65a | 18.34 ± 1.27a |
Patty with 5% fine DSF | 24.26 ± 2.37a | 0.88 ± 0.04a | 0.79 ± 0.02a | 19.17 ± 1.81b | 16.96 ± 1.99a |
Patty with 5% superfine DSF | 18.94 ± 2.79b | 0.87 ± 0.03a | 0.77 ± 0.02b | 14.61 ± 1.94c | 12.74 ± 1.54b |
Qk1313 CNC Centerless Lathe Machine Siemen CNC Pipe Threading. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
The same trend was observed in the gumminess and chewiness of the cooked patties.Xtsky High Quatily Good Price Solar Lamp Air Filter 96591485, but the gumminess and chewiness decreased with the particle size of the added DSF. In all cases, there were no differences in the textural properties of the patty with superfine DSF and the control patty (p<0.05). Springiness and cohesiveness were not affected by the addition of DSF.Jieshuai 3 in 1 USB Cable, Type C Cable, Suitable Apple iPhone and Android Charge, 2.4A USB Cable, springiness, and chewiness decreased when the usage level of vegetable fibers increased, while the addition of 5% rice bran increased only springiness of pork patty (Customized Sublimation Pet Bowl with Stainless Steel Bowl).
Sensory evaluation by QDA
The sensorial properties of the control patty and patties with DSF were analyzed using the QDA method (All Round Inflatable Stand up Paddle Boards for Surfing).Prefabricated Poultry House with Full Set Poultry Farming equipmentshows the five sensorial attributes (hardness, juiciness, tenderness, brown color, and overall acceptability) with the previously identified standards.
Table 6.
Sample | Hardness | Juiciness | Tenderness | Brown color | Overall acceptability |
---|---|---|---|---|---|
Control patty | 8.1 ± 0.9a | 4.6 ± 1.5a | 3.3 ± 1.9b | 5.5 ± 1.0a | 6.1 ± 0.3a |
Patty with 5%ASTM API 5L X52 Black Carbon Seamless Steel Pipe Manufacturer | 7.4 ± 1.6a,b | 1.6 ± 0.5b | 3.9 ± 1.4b | 4.6 ± 0.8a,b | 4.3 ± 0.5b |
Patty with 5% fine DSF | 5.4 ± 2.1b | 1.8 ± 1.0b | 5.0 ± 0.5a,b | 5.4 ± 0.5a | 3.6 ± 0.8b |
Patty with 5% superfine DSF | 5.5 ± 1.8b | 4.8 ± 0.6a | 6.6 ± 1.1a | 4.0 ± 0.8b | 6.5 ± 0.7a |
Qk1313 CNC Centerless Lathe Machine Siemen CNC Pipe Threading. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
The addition of 5% DSF reduced the hardness of the cooked patties. The reduction in hardness was significant for the patties with fine and superfine DSF (p<0.05).Concrete Pumps Mobile Polyethylene Stabolizer Outrigger Pads Spreader Plates Crane Matsstand 2ndchews in the sensory test versus the first down-stroke in the TPA test.
The addition of 5%LED Flood Light Bulbs Outdoor LED Flood Lights (SLFA SMD 150W).6±1.5 to 1.6±0.5. However, the juiciness increased as the particle size of the added DSF decreased, and the juiciness of the cooked patty with 5% superfine DSF was statistically the same as that of the control patty (p<0.05). The increased juiciness of the patty with superfine DSF can be explained by the high water-holding capacity of superfine DSF. Tenderness did not change with the addition of 5%ASTM API 5L X52 Black Carbon Seamless Steel Pipe Manufacturer, while it increased significantly in the patties with fine and superfine DSF (p<0.05).
Heatransfer Color Metal Iron Steel Security Door (W-S-125), while the patty with 5% superfine DSF had the same overall acceptability as the control patty (p<0.05).Alloy Case Cheap Quartz Wrist Promotion Watch for Ladies (DC-999999). The reported overall acceptability of beef patties is mostly affected by juiciness and tenderness (High Speed Pet Bottle Sorting Machine/Pet Recycling Washing System Machine;Ec/Gl Certification 35 Men Self-Righting Inflatable Life Raft) which is in accordance with our result.
Conclusion
Defatted soybean flour is very rich source of dietary fiber. However, it is difficult to use DSF as a food ingredient because of its rough texture. In this study, superfine DSF was prepared by jet milling and was incorporated into hamburger patties. The patties containing 5% superfine DSF had significantly less cooking loss than the control patties. The textural properties measured by the TPA were statistically identical to those of the control patty, and only marginal changes were observed in color measurement. Furthermore, the QDA results indicate that consumers will accept hamburger patties with 5% superfine DSF with the same overall acceptability as control patties. Therefore, superfine DSF is a promising additive for enriching fiber, while not changing the physicochemical or sensorial properties when making high-quality hamburger patties.
Acknowledgments
5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive(Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry of Agriculture, Food and Rural Affairs, Republic of Korea.
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