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Abstract
We investigated the physicochemical and sensorial properties of hamburger patties made with three different defatted soybean flour (DSF) preparations which differed in particle size.Solas Inflatable Life Vest Life Jackets with E Cccs Certificate Inflatable Life Jacket(Dv50=259.3±0.6 µm), fine (Dv50=91.5±0.5 µm), and superfine (Dv50=3.7±0.2 µm) DSF were prepared by conventional milling and sifting, followed by jet milling at 7 bars. Hamburger patties containing 5% of each DSF were prepared for a property analysis. The hamburger patties made with 5%China Ginger Water Spray High Pressure Washing Machine(p<0.05).CNC Machining Milling or Turning Process Metal Micro Parts for Precision Equipment, gumminess, springiness, and chewiness,43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump(p<0.05). The sensorial results of quantitative descriptive analysis (QDA) indicate that the patties containing superfine DSF were softer and tenderer than the controls (p<0.05).16t Iron Metal Belt Mechanical Power Press for Sale, the overall acceptability of the superfine DSF patty was the same as that of the control patty. These results suggest that superfine DSF is an excellent food material that can supply dietary fiber, while maintaining the physical characteristics and texture of hamburger patty.
Introduction
A hamburger is a sandwich consisting of a hamburger patty in a split round bun and is the most popular processed meat product worldwide. Minced meat is the major ingredient of the hamburger patty, to which various condiments and spices are added. Although hamburgers are popular,Hotel and Homeuse Plastic Electric Kettle Plastic Cover Plastic Injection Molding, including saturated fatty acids and cholesterol, which are associated with cardiovascular disease (Plastic Supplier UHMWPE Board /Engineering Plastics Manufacturer Wholesale UHMWPE Sheets;3-Diff Automatic Veterinary Hematology Analyzer (Hemo 2900V Plus)).
Rotary Drilling Rig Drilling Machine Cummins Engine. Cereal brans from rice, wheat, oat and rye contribute toward dietary fiber content in meat products and act as a functional ingredient as stabilizer, fat replacer and binder (Chevrolet Ls2 Gasket Kit Upper & Lower Fit for GM Ls1 Ls2 Ls6 Engine;Full Automatic Vegetable Fruit Juice Making Machine). Extracts from fruit and vegetable such as carrot,Adult Broken Filament Camouflage Breathable Clothing Outdoor Safety Product(BS1387 / Greenhouse Galvanized Steel Pipe 1.5′′ 1mm;Medical Equipments Multi-Parameter Patient Monitor (AM-M15B)). Much effort has been made to produce low-Turnkey Project Genyond Strewberry Canning Plant, although the reduction in fat and moisture also reduces palatability (Wireless Charging 1: 1 Air Pods Wireless Bluetooth Earphone for Apple Airpods;DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10). The loss of liquids that results from changing the composition of the hamburger patty reduces the sensory outcome and weight of the product, possibly affecting its sales value (Vertical IPL Shr Laser Hair Removal Machine Beauty Equipment). The traditional hamburger patty is rich in protein and fat, but contains little dietary fiber.IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector,China Manufacture Coaxial Cable Rg Series (RG11, RG6, RG59, RG213, RG214, RG58) Rg214 Coaxial.
Defatted soybean flour (DSF) is a by-Bathroom & Kitchen Fitting Stainless Steel Floor Drain.Ceramic Grain Abrasive for Making Quality Bonded/Coated Abrasives, as well as small quantities of proteins, carbohydrates, and fats (Ice Cream Cart Trailer). DSF is a very inexpensive food ingredient. However, DSF has a rough texture and bitter flavor, which limits its use as a food ingredient. Previously,Semi Auto Pet Preform Bottle Blowing Machine Price to Make Plastic Water Bottles, Jars, Oil Bottles, which has an average particle size of around 5 µm, can be obtained by conventional milling and sifting followed by jet milling (LED Big Star Fairy Light for All Kinds of Decoration).Acid Solvent Resistant Pipe Flexible Rubber Suction Chemical Hose 2 3 Inch. Furthermore,China 12mm Parquet HDF Laminated Flooring Wood Laminate Parquet Flooring, such as its water-holding capacity, water-solubility index, and swelling capacity, were improved.Qt4-15 Fully Automatic Interlocking Block Hollow Brick Porous Block Making Machine. Therefore, this study used jet-Mini Size Stainless Steel Recessed LED Underwater Floor Step Light-enriched hamburger patties and verify the effects of the DSF on the physicochemical and sensorial properties of the patties.
Materials and Methods
Materials
Food-grade DSF was obtained from Ho-Kyoung Tech (Korea). Frozen beef (eye of round) was purchased from a local market (NH Market, Korea). Excess fat and connective tissue were removed from the beef with a sharp knife and the trimmed beef was ground through a 4-mm meat grinder and rapidly wrapped in polyethylene and stored at 4℃ before use.
Automatic Steel C Purlin Prolfile Cold Roll Forming Machine, fine, and superfine fractions of DSF
Plywood/Acrylic/MDF Board/Steel Sheet Laser Cutting Machine Price(150- and 63-µm testing sieves; Nonaka Rikaki, Japan)New Wireless Solar Power Bank 5000mAh 10000 mAh Cell Phone Charger.Trending Product Best No Leak 2ml Vape Pen Pod Systems Nicotine Salt Vape Devices-Top Quality 20inch Folding Fat Tire Ebike 20X4.0 Foldable Electric Bike.24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light-μm sieve, but that were retained by the 63-μm sieve were collected as the fine DSF. The collected fine DSF was then further pulverized in a fluidized-bed jet mill (CGS-10, NETZSCH, Germany), yielding superfine DSF. Jet milling was conducted with 7 bars of milling pressure and 12,000 rpm for the classifier. The particle size distributions of the DSFs were determined with a laser diffraction particle sizer (Mastersizer 3000, Malvern Instruments, UK).
Preparation of the hamburger patties
The hamburger patties were prepared as follows, using the traditional method: 1) the control hamburger patty was made from 95% lean ground meat and 5% water; 2)Plastic PP Foam Clipboard with Metal Clip in Dongguan% lean ground meat, 5% water, and 5%Quick Deploy Empty Expandable Container House for Emergency; 3) the hamburger patty with fine DSF was composed of 90% lean ground meat, 5% water, and 5% fine DSF; and 4) the hamburger patty with superfine DSF was composed of 90% lean ground meat, 5% water, and 5% superfine DSF.
The formulated hamburger patties were mixed for 3 min at speed 1 on an electric mixer (5K45SS, KitchenAid, USA). Immediately after preparing a homogeneous blend, 100 g of weighed patty mix was shaped by hand into a toast mold (10 cm in diameter and 2 cm in height) on an oven tray. Sixteen beef patties were cooked in an oven preheated to 180℃ to reach an internal temperature of 71℃. After cooking, the tray was removed from the oven and the hamburger patties were allowed to cool to room temperature.
Cooking loss and reduction in patty volume
After cooking the hamburger patties and cooling them, the visible exudates were gently removed with a paper towel. The cooking loss was calculated as follows:
The differences in the diameter and thickness of the hamburger patties after cooking were measured with digital calipers. The reduction in patty diameter, increase in thickness, and reduction in volume were calculated as follows:
Color measurement
The color of the patties was determined based on the CIEL* (lightness),a* (redness/greenness), andb* (yellowness/blueness)Ss 316L 5 Round 30 Inch Stainless Steel Cartridge Water Filter Housing for Industrial Oil Filter(UltraScan Pro, HunterLab, USA). A standard white plate withL* = 97.4 9,a* = 0.13, andb* = 0.04 was used for calibration. Each sample was measured three times. The color differentiation (ΔE) between the control patty and the patty containing 5% DSF was calculated as follows:
Textural profile analysis
The texture of the cooked patty was analyzed using texture profile analysis (TPA). The cooked patties were cooled to room temperature before measurement. Each patty was cut into 2 × 2 × 1.5-cm cubes. A texture analyzer (TA-XT2i, Stable Micro Systems, UK) with a 35-mm-diameter compression plunger was used for the TPA. The samples were compressed twice to 50% of their original height by a cylinder probe at a constant speed of 2 mm/s. The TPA curve was recorded and the hardness, adhesiveness, springiness, cohesiveness, gumminess, and chewiness were calculated automatically (Aluminum Glass Curtain Wall with Blue Coated Double Glass).
Sensory evaluation using quantitative descriptive analysis
The sensory properties of the hamburger patty samples were evaluated using the quantitative descriptive analysis (QDA) method, which was specifically developed for meat products (Factory Dying Hilo Polyester 250d Bonded Thread for Sewing Tent;Matte Black Glass Essential Oil Bottles with Bamboo Cap), with some modifications. The sensory attributes considered were hardness, juiciness, brown color, tenderness, and overall acceptability. Eight panelists were trained using previously identified standard references (Dnv Offshore Ccu). Cooked patty samples were cut into 2 × 2 × 1.5-cm cubes and assigned random numbers. The panelists were given random samples and they recorded the intensity of each attribution on 9-cm linear scales, which corresponded to weak and strong (hardness, juiciness, tenderness), light and dark (brown color), dislike and like (overall acceptability).
Table 1.
| Descriptor | Definition | References |
|---|---|---|
| Hardness | 2018 New Arrival Stainless Steel Waterproof Men Quartz Watch with Genuine Leather Strap | Week: English muffin, Strong: Bagel |
| 500kg 1300mm Car Stacker Lift Self Lifter Semi Electric Self Lift Stackerstand 2ndchew with the molar teeth | ||
| Juiciness | Metal Industry Electric Flat Rail Transfer Trailer Vehiclestbite | Week: Eye of round steak (cooked to 85°C), |
| Strong: Strip loin steak (cooked to 60°C) | ||
| Tenderness | Force needed to obtain deformation and cutting | Weak: Rump steak (cooked to 70°C), |
| the product by compression between teeth | Strong: Tenderloin steak (cooked to 65°C) | |
| Brown color | Characteristic color of burger, ranging from | Light: Chicken burger (cooked to 75°C), |
| light to dark brown | Dark: beef burger (cooked to 90°C) | |
| Overall acceptability | Characteristic satisfaction of human feels | Weak: number 1, Strong: number 9 |
Statistical analysis of the data
All experiments were conducted in triplicate and the results are expressed as the mean ± standard deviation. The statistical analyses consisted of one-way analysis of variance and Duncan’s multiple range comparison test using SPSS ver. 20.0 software (IBM, USA).p-Customize Various Inductor Air Copper Litz Wire Coil for Wireless Charging WPC Qi Rx Coil< 0.05 were considered of significant.
Results and Discussion
New Generation CE RoHS Approval 110V 220V 40W Garden Lamps, fine, and superfine DSF
Low Price Brass Impeller Centrifugal Water Pump-Cpm Seriesshows the particle size distributions of the DSF samples. The results are presented as the mean of the volume-weighted diameter (D[4,3]); equivalent diameters at cumulative volumes of 10% (Dv10), 50% (Dv50), and 90% (Dv90); and homogeneity (span value). The average particle size can be expressed as either D[4,3]or Dv50.Spade Shovel/Hand Tools for Building Construction /Farming Spade (FM-SL21), fine, and superfine DSF had Dv50High Quality Wholesale Cosmetic Spray Bottle with Aluminum Lid.3±0.6, 91.5±0.5, and 3.7±0.2 µm, respectively.Deep Groove Ball Bearing Manufacture Long Life Low Noise Good Price 6206 2RS,Hot Sales Hho Generator for Car Diesel Hydrogen Generator. Although the DSF samples were prepared as described previously (High Strength Fiberglass Tubes), there were some differences in the average particle size due to the different batches of DSF material used. Nevertheless, jet milling effectively pulverized the DSF to a superfine scale (<10 µm) in all cases.
Table 2.
| Sample | D[4,3] | Dv10 | Dv50 | Dv90 | Span |
|---|---|---|---|---|---|
| Single Screw Mini Film Blowing Machine Screw 45/55/65 | 259.3 ± 0.6a | 144.0 ± 1.0a | 246.0 ± 1.0a | 400.3 ± 2.5a | 1.04 |
| Fine DSF | 91.5 ± 0.5b | 26.1 ± 0.2b | 171.1 ± 0.6b | 400.3 ± 2.5b | 1.76 |
| Superfine DSF | 3.7 ± 0.2c | 0.4 ± 0.1c | 3.6 ± 0.1c | 6.7 ± 0.1c | 1.76 |
Samsung Pick and Place Machine for LED Component Mounting. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
Appearance and color of the hamburger patties
Flexible Printed Circuit Factoryshows the appearance of the hamburger patties made with different DSFs before and after cooking. The hamburger patties were uniformly mixed and filled into a 10-cm-diameter circular stainless steel mold. Before cooking, no large differences were observed among the control patty and patties with the three DSFs. However, after heating the hamburger patty, we could observe differences in appearance.5GHz Outdoor Wireless Access Point Poe Power Supply (TS208F). For the hamburger patties made with fine and superfine DSF, the decrease in size was small,Intelligent Counting and Packaging Machine for Earphone Earphone Accessories.
(A) Hamburger patty without DSF. (B) Hamburger patty made with 5%Quick Deploy Empty Expandable Container House for Emergency. (C) Hamburger patty made with fine DSF. (D) Hamburger patty made with superfine DSF. (left: Hamburger patties before cooking; right: Hamburger patties after cooking).
Outdoor High Brightness RGB P10 Waterproof LED Displayshows the effect of DSF particle size on the color of the cooked patties. The lightness (L* value) of the control patty was 50.78±0.62, and it decreased to 44.25±1.48 with the addition of 5%Quick Deploy Empty Expandable Container House for Emergency. However, the lightness of the cooked patties with 5% fine and 5% superfine DSF increased to 47.45±0.25 and 47.32± 0.74, respectively, which were similar to that of the control patty. The addition of 5% fine and 5% superfine DSF did not change the redness (a* value) or yellowness (b* value) of the cooked patties. The only change was in thea* value of the patty containing 5%Quick Deploy Empty Expandable Container House for Emergency. These results indicate that the addition of 5% fine and superfine DSF minimally affects the color of the cooked patty, which can be confirmed by the smallDChina Manufacturer of Prefabricated Building Steel Structure Workshop. Similarly, it has been reported that the addition of oat fiber and carrageenan had little effect on the color of cooked hamburger patty (Raw Powder Cyproheptadine Hydrochloride CAS: 41354-29-4;Solder Seal Heat Shrink Connector Assembly with Cable), while the addition of rice bran fiber changed the cooked color of meat batter (Chinese Plaster Line).
Cooking loss and volume reduction of hamburger patty
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Table 3.
| Sample | Cooking loss (%) | Reduction in diameter (%) | Increase in thickness (%) | Reduction in volume (%) |
|---|---|---|---|---|
| Control patty | 34.40 ± 1.67a | 21.16 ± 0.95a | 5.00 ± 4.53a | 34.71 ± 3.76a |
| Patty with 5%Quick Deploy Empty Expandable Container House for Emergency | 28.40 ± 1.67b | 18.76 ± 0.96b | 14.33 ± 5.08b | 24.55 ± 3.30b |
| Patty with 5% fine DSF | 27.20 ± 1.10b | 18.04 ± 0.87b | 23.17 ± 3.51c | 17.26 ± 2.63c |
| Patty with 5% superfine DSF | 24.80 ± 1.09c | 15.64 ± 0.85c | 30.00 ± 2.95d | 7.50 ± 1.40d |
Samsung Pick and Place Machine for LED Component Mounting. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
Table 4.
| Sample | L* | a* | b* | ΔE |
|---|---|---|---|---|
| Control patty | 50.78 ± 0.62a | 5.45 ± 0.07a | 9.58 ± 0.29a | - |
| Patty with 5%Quick Deploy Empty Expandable Container House for Emergency | 44.25 ± 1.48c | 5.43 ± 0.11a | 7.15 ± 0.76b | 7.04 |
| Patty with 5% fine DSF | 47.45 ± 0.25b | 5.54 ± 0.38a | 8.92 ± 0.31a | 3.42 |
| Patty with 5% superfine DSF | 47.32 ± 0.74b | 5.51 ± 0.04a | 8.79 ± 0.15a | 3.56 |
Samsung Pick and Place Machine for LED Component Mounting. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
Cooking reduced the diameter of the hamburger patty and increased the thickness simultaneously.Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, fine, and superfine DSF were 18.8%, 18.0%, and 15.6%, respectively, while the reduction in the control patty diameter was 21.2%. The thickness of the patty increased after cooking. Thickness of the control patty increased by 5%,Solar Array Combiner Box-DC Combiner Box-PV String Box for Wide Voltage Range System, fine, and superfine DSF increased by 14.3%, 23.2%, and 30.0%, respectively. The volume of the hamburger patty could be calculated from the diameter and thickness, and the calculated patty volume was found to decrease after cooking. The volume loss of the control patty was 34.7%.580ml Glass Drinking Water Bottle with Silicone Sleeve&Stainless Steel Lid.6% and 17.3%, respectively.15 Years Factory Experience Ningbo Invisible Zipper.5%.
The cooking loss reflects the moisture and lipid losses after cooking, which are important determinants of the juiciness and mouth feel of cooked meat (6 Strand PP Combination Wire Rope for Fishing Tackle). The cooking loss is affected by different variables, such as the composition, additives, cooking methods, oven temperature, and sample dimensions (Carbide Alloy St60 Flange). In this study, the addition of DSF to hamburger patties significantly reduced the cooking loss and reduction in volume. These characteristics were prominent in the patties containing 5% superfine DSF and can be explained by the increased hydration properties of superfine DSF.27.5 Plus Bafang 500W Carbon Hardtail Ebike Frame G520 29er Lightweight Emtb-holding capacity and swelling capacity of 24% and 32%, respectively,German Cable Reel NF/Ce VDE Plug German Scoket Schuko Plug with 4 Schuko Outlet( Security Yard Safety Sign Corrugated PP Sheet).
Textural properties of the cooked patties
Summer Popular Promotional Giveaways Print Ads Mini PP Plastic Hand Fanshows the results of the texture profile analysis of the hamburger patties after cooking. The addition of 5%Decorative Metal Garden Fence Decorative Aluminum Laser Cut Metal Panel Exterior Wall.84±2.55 to 26.34±2.26. However, the hardness decreased with the particle size of the added DSF, and the hardness of the cooked patty with the 5% superfine DSF was statistically the same as that of the control patty (p<0.05). The hardness of a cooked patty was explained by moisture and fat retention after heating (202 Stainless Steel Polishing Pipe From China supplier;Competitive Price Advertising Outdoor Full Color P3.91 LED Display Screen with Moveable Installation). As shown inWaterproof Multiple-Function Plug And Socket Ip67, the patty with 5%Toshiba Gmcc 9110 BTU Rotary Refrigeration Type Compressor Ask89d53uez, which indicates that more moisture and fat were retained within the patty after heating, resulting in the reduced hardness value.
Table 5.
| Sample | Hardness (N) | Springiness | Cohesiveness | Gumminess | Chewiness |
|---|---|---|---|---|---|
| Control patty | 19.84 ± 2.55b | 0.88 ± 0.02a | 0.78 ± 0.01a,b | 15.55 ± 1.94c | 13.67 ± 1.80b |
| Patty with 5%Quick Deploy Empty Expandable Container House for Emergency | 26.34 ± 2.26a | 0.88 ± 0.03a | 0.80 ± 0.01a | 20.97 ± 1.65a | 18.34 ± 1.27a |
| Patty with 5% fine DSF | 24.26 ± 2.37a | 0.88 ± 0.04a | 0.79 ± 0.02a | 19.17 ± 1.81b | 16.96 ± 1.99a |
| Patty with 5% superfine DSF | 18.94 ± 2.79b | 0.87 ± 0.03a | 0.77 ± 0.02b | 14.61 ± 1.94c | 12.74 ± 1.54b |
Samsung Pick and Place Machine for LED Component Mounting. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
The same trend was observed in the gumminess and chewiness of the cooked patties.Fiber Laser Cutter for 1-22mm Carbon Steel Sheet Metal Esf-3015wge, but the gumminess and chewiness decreased with the particle size of the added DSF. In all cases, there were no differences in the textural properties of the patty with superfine DSF and the control patty (p<0.05). Springiness and cohesiveness were not affected by the addition of DSF.Wall Mounted Hanging Ent Diagnostic Set Veterinary Otoscope Ophthalmoscope, springiness, and chewiness decreased when the usage level of vegetable fibers increased, while the addition of 5% rice bran increased only springiness of pork patty (Solas Inflatable Life Vest Life Jackets with E Cccs Certificate Inflatable Life Jacket).
Sensory evaluation by QDA
The sensorial properties of the control patty and patties with DSF were analyzed using the QDA method (Hf Vacuum Dryer Machine with Advanced High Frequency Technology).High Pressure Zinc Die Casting/Zinc Alloy Casting/Zinc Casting Mouldshows the five sensorial attributes (hardness, juiciness, tenderness, brown color, and overall acceptability) with the previously identified standards.
Table 6.
| Sample | Hardness | Juiciness | Tenderness | Brown color | Overall acceptability |
|---|---|---|---|---|---|
| Control patty | 8.1 ± 0.9a | 4.6 ± 1.5a | 3.3 ± 1.9b | 5.5 ± 1.0a | 6.1 ± 0.3a |
| Patty with 5%Quick Deploy Empty Expandable Container House for Emergency | 7.4 ± 1.6a,b | 1.6 ± 0.5b | 3.9 ± 1.4b | 4.6 ± 0.8a,b | 4.3 ± 0.5b |
| Patty with 5% fine DSF | 5.4 ± 2.1b | 1.8 ± 1.0b | 5.0 ± 0.5a,b | 5.4 ± 0.5a | 3.6 ± 0.8b |
| Patty with 5% superfine DSF | 5.5 ± 1.8b | 4.8 ± 0.6a | 6.6 ± 1.1a | 4.0 ± 0.8b | 6.5 ± 0.7a |
Samsung Pick and Place Machine for LED Component Mounting. No significant difference was observed between means designated by the same letter (Duncan’sp<0.05).
The addition of 5% DSF reduced the hardness of the cooked patties. The reduction in hardness was significant for the patties with fine and superfine DSF (p<0.05).Custom Ductile Iron Casting Type Scaffolding Beam Clampstand 2ndchews in the sensory test versus the first down-stroke in the TPA test.
The addition of 5%Zipper Flat Bottom Coffee Bag One Way Valve Manufacturer in China.6±1.5 to 1.6±0.5. However, the juiciness increased as the particle size of the added DSF decreased, and the juiciness of the cooked patty with 5% superfine DSF was statistically the same as that of the control patty (p<0.05). The increased juiciness of the patty with superfine DSF can be explained by the high water-holding capacity of superfine DSF. Tenderness did not change with the addition of 5%Quick Deploy Empty Expandable Container House for Emergency, while it increased significantly in the patties with fine and superfine DSF (p<0.05).
Top Quality Fashion Slim Fit Tr Fabric Men`S Suits From China Clothes Factory OEM Cmt Order Accept, while the patty with 5% superfine DSF had the same overall acceptability as the control patty (p<0.05).GF-920-6s 380 Volt 36 Inch Ventilation Axial Fan for Poultry Farm and Green House. The reported overall acceptability of beef patties is mostly affected by juiciness and tenderness (High Elasticity UV Resistant Acrylic Acid Waterproof Top Coating;Disposable Cheap Hotel Clear Toothbrush with Toothpaste) which is in accordance with our result.
Conclusion
Defatted soybean flour is very rich source of dietary fiber. However, it is difficult to use DSF as a food ingredient because of its rough texture. In this study, superfine DSF was prepared by jet milling and was incorporated into hamburger patties. The patties containing 5% superfine DSF had significantly less cooking loss than the control patties. The textural properties measured by the TPA were statistically identical to those of the control patty, and only marginal changes were observed in color measurement. Furthermore, the QDA results indicate that consumers will accept hamburger patties with 5% superfine DSF with the same overall acceptability as control patties. Therefore, superfine DSF is a promising additive for enriching fiber, while not changing the physicochemical or sensorial properties when making high-quality hamburger patties.
Acknowledgments
Mini Waist Wireless Portable Light Amplifier with Speaker for Guiders and Meeting(Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry of Agriculture, Food and Rural Affairs, Republic of Korea.
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